recombinant human gdnf protein r d systems (R&D Systems)
96
Structured Review
R&D Systems
recombinant human gdnf protein r d systems
Recombinant Human Gdnf Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+gdnf+protein+r+d+systems/Recombinant+Human+GDNF+Protein/pm41579373-615-174-178
Average 96 stars, based on 288 article reviews
Recombinant Human Gdnf Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+gdnf+protein+r+d+systems/Recombinant+Human+GDNF+Protein/pm41579373-615-174-178
Average 96 stars, based on 288 article reviews
recombinant human gdnf protein r d systems - by Bioz Stars,
2026-10
96/100 stars
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Recombinant:Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 RNA Sequencing:Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Software:Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 SYBR Green Assay:Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Single Cell:Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 cDNA Synthesis:Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Quantitative RT-PCR:Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Gene Expression:Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 |