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recombinant human gdnf protein r d systems  (R&D Systems)


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    R&D Systems recombinant human gdnf protein r d systems
    Recombinant Human Gdnf Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+gdnf+protein+r+d+systems/Recombinant+Human+GDNF+Protein/pm41579373-615-174-178
    Average 96 stars, based on 288 article reviews
    recombinant human gdnf protein r d systems - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Recombinant Human GDNF Protein R&D Systems Cat#212-GD Lonafarnib (SCH66336) Sellekchem Cat#S2797 Salirasib Sellekchem Cat#S7684 Deposited data RNA sequencing data This paper GSE297876 Oligonucleotides See Table S3 for a list of primers N/A N/A Software and algorithms STAR (v2.5.2b) Dobin et al. 201351 https://github.com/alexdobin/STAR SubRead (featureCounts) Liao et al. 201452 http://subread.sourceforge.net DESeq2 Love et al. 201453 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html pheatmap (R package) Kolde 202554 https://cran.r-project.org/web/packages/ pheatmap/index.html Metascape Zhou et al. 201955 https://metascape.org Fiji (ImageJ) Schindelin et al. 201256 https://imagej.net/software/fiji/ SigmaPlot v14.0 Systat Software https://systatsoftware.com/products/sigmaplot/ GraphPad Prism v9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R v4.3.1 R Core Team https://www.r-project.org Cell Reports 45, 116868, February 24, 2026 17 ..

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    RNA Sequencing:

    Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Recombinant Human GDNF Protein R&D Systems Cat#212-GD Lonafarnib (SCH66336) Sellekchem Cat#S2797 Salirasib Sellekchem Cat#S7684 Deposited data RNA sequencing data This paper GSE297876 Oligonucleotides See Table S3 for a list of primers N/A N/A Software and algorithms STAR (v2.5.2b) Dobin et al. 201351 https://github.com/alexdobin/STAR SubRead (featureCounts) Liao et al. 201452 http://subread.sourceforge.net DESeq2 Love et al. 201453 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html pheatmap (R package) Kolde 202554 https://cran.r-project.org/web/packages/ pheatmap/index.html Metascape Zhou et al. 201955 https://metascape.org Fiji (ImageJ) Schindelin et al. 201256 https://imagej.net/software/fiji/ SigmaPlot v14.0 Systat Software https://systatsoftware.com/products/sigmaplot/ GraphPad Prism v9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R v4.3.1 R Core Team https://www.r-project.org Cell Reports 45, 116868, February 24, 2026 17 ..

    Software:

    Article Title: Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti Human C19ORF81 Novus Biological Cat# NBP2-32518; RRID:AB_3282455 Rabbit polyclonal anti Human C19ORF84 Atlas Antibodies Cat# HPA068082; RRID:AB_2685949 Rabbit polyclonal anti Human CAMK2B Novus Biologicals Cat# NBP1-88212; RRID:AB_11016092 Rabbit polyclonal anti Human CCK Novus Biologicals Cat# NBP2-62664; RRID:AB_3353182 Mouse monoclonal anti Human CD82 Proteintech Cat# 66803-1-Ig; RRID:AB_2882146 Rabbit polyclonal anti Human CDH15 Novus Biologicals Cat# NBP1-88101; RRID:AB_11019082 Rabbit polyclonal anti Human CELF4 GeneTex Cat# GTX121390; RRID:AB_11178502 Mouse monoclonal anti Human CELF4 DHSB Cat# PCRP-CELF4-1C3; RRID:AB_2618496 Mouse monoclonal anti Human CTSG MyBioSource Cat# MBS555070; RRID:AB_3699235 Rabbit polyclonal anti Human CTSG Boster Bio Cat# PB10053; RRID:AB_3699237 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C820482; RRID:AB_3699236 Rabbit polyclonal anti Human EGR4 LifeSpan BioSciences Cat# LS-C402257; RRID:AB_2782963 Rabbit polyclonal anti Human ENO3 LifeSpan BioSciences Cat# LS-C211358; RRID:AB_3699238 Mouse monoclonal anti Human FGFR3 Novus Biologicals Cat# NBP2-52468; RRID:AB_2782964 Mouse monoclonal anti Human FGFR3 Thermo Fisher Scientific Cat# MA5-26493; RRID:AB_2723178 Rabbit polyclonal anti Human FSD1 GeneTex Cat# GTX131769; RRID:AB_2782962 Rabbit polyclonal anti Human GAL Novus Biologicals Cat# NBP2-33582; RRID:AB_3285153 Goat polyclonal anti Human GFRA1 R&D Systems Cat# AF714; RRID:AB_355541 Rabbit polyclonal anti Human ITLN1 MyBioSource Cat# MBS200265; RRID:AB_3699285 Rabbit polyclonal anti Human ITLN1 Proteintech Cat# 11770-1-AP; RRID:AB_2129677 Rabbit polyclonal anti Human ITLN1 Elabscience Cat# E-AB-68401; RRID: AB_3699286 Mouse monoclonal anti Human MKI67 Novus Biologicals Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT R&D Systems Cat# AF332; RRID:AB_355302 Mouse monoclonal anti Human NANOS2 LifeSpan BioSciences Cat# LS-C73159-100; RRID:AB_1599930 Mouse monoclonal anti Human NANOS2 Santa Cruz Biotechnology Cat# sc-393868; RRID:AB_3699234 (Continued on next page) 16 Cell Reports 45, 116868, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti Human NANOS3 Proteintech Cat# 21679-1-AP; RRID:AB_1085925 Rabbit polyclonal anti Human NANOS3 Novus Biologicals Cat# NBP1-76919; RRID:AB_11022652 Rabbit polyclonal anti Human PIWIL4 LifeSpan BioSciences Cat# LS-C482396-50; RRID:AB_2782961 Rabbit polyclonal anti Human PLPPR3 Atlas Antibodies Cat# HPA057034; RRID:AB_2683316 Mouse monoclonal anti Human PIWIL4 Novus Biologicals Cat# NBP2-3739; RRID:AB_10859250 Mouse monoclonal anti Human PIWIL4 GeneTex Cat# GTX60725; RRID:AB_3699239 Rabbit polyclonal anti Human PPP1R36 Novus Biologicals Cat# NBP2-62718; RRID:AB_3353233 Rabbit polyclonal anti Human SH2B2 LifeSpan BioSciences Cat# LS-C805414; RRID:AB_3699288 Rabbit polyclonal anti Human SOCS1 GeneTex Cat# GTX100657; RRID:AB_1951971 Rabbit polyclonal anti Human TMEM181 Novus Biologicals Cat# NBP1-93711; RRID:AB_1100592 Rabbit polyclonal anti Human TMEM181 Proteintech Cat# 55411-1-AP; RRID:AB_11182284 Mouse monoclonal anti Human UCHL1 GeneTex Cat# GTX34969; RRID:AB_3699240 Biological samples Testicular biopsies from 10 fertile men (Age 25–50 yrs) UCSD N/A Testicular biopsies from 9 fertile men (aged 27, 28, 30, 34, 38, 38, 40, 40, and 46) University of Pittsburgh Health Sciences Tissue Bank and Center for Organ Recovery N/A Chemicals, peptides, and recombinant proteins Human Recombinant bFGF Thermo Fisher Cat#NC1085507 Recombinant Human GDNF Protein R&D Systems Cat#212-GD Lonafarnib (SCH66336) Sellekchem Cat#S2797 Salirasib Sellekchem Cat#S7684 Deposited data RNA sequencing data This paper GSE297876 Oligonucleotides See Table S3 for a list of primers N/A N/A Software and algorithms STAR (v2.5.2b) Dobin et al. 201351 https://github.com/alexdobin/STAR SubRead (featureCounts) Liao et al. 201452 http://subread.sourceforge.net DESeq2 Love et al. 201453 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html pheatmap (R package) Kolde 202554 https://cran.r-project.org/web/packages/ pheatmap/index.html Metascape Zhou et al. 201955 https://metascape.org Fiji (ImageJ) Schindelin et al. 201256 https://imagej.net/software/fiji/ SigmaPlot v14.0 Systat Software https://systatsoftware.com/products/sigmaplot/ GraphPad Prism v9 GraphPad Software https://www.graphpad.com/scientific- software/prism/ R v4.3.1 R Core Team https://www.r-project.org Cell Reports 45, 116868, February 24, 2026 17 ..

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    SYBR Green Assay:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Single Cell:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    cDNA Synthesis:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Quantitative RT-PCR:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Gene Expression:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS



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